محلول 20 میلیگرمی پروتئیناز K

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محلول 20 میلیگرمی پروتئیناز K

Proteinase K Solution (20 mg/ ml) پروتئیناز کا

Format: solution

Molecular Biology

Volume: 1 ml, 20 mgr,

Cat#: DB9682-20mg

DNAbiotech

Storage: -20^oC

For Molecular Reasearch Use

www.kalazist.ir

Proteinase K from the fungus Engyodontium album is a nonspecific serine protease that is useful for general digestion of proteins

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Description:

What is proteinase K used for?

Proteinase K is a broad spectrum serine protease, used in many DNA extraction protocols to digest these contaminating proteins. In addition, there may be nucleases (enzymes that degrade nucleic acids) present.

What does proteinase K do to the cell?

This enzyme used for the destruction of proteins in cell lysates (tissue, cellculture cells) and for the release of nucleic acids, since it very effectively inactivates DNases and RNases.

How does Pr. K would be deactivated?

Heat is a widely used way of inactivating proteinase K. While the activity ofproteinase K increases with temperature, and is optimized at about 65 ˚C, heating proteinase K to 95 ˚C for 10 minutes will inactivate it. Keep in mind, however, that heating proteinase K does not fully inactivate the enzyme

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